1183 Expression of adhesion molecules and basic fibroblast growth factor 2 in non-segmental vitiligo-derived primary melanocytes

Primary melanocytes isolated from non-depigmented skin of two Japanese patients with non-segmental vitiligo were difficult to grow in culture. Adding a ROCK inhibitor the culture medium dramatically increased yield pure melanocytes. RT-qPCR analysis vitiligo-derived primary revealed significantly decreased MITF mRNA levels compared normal In contrast, SCF expression higher co-cultures and keratinocytes than keratinocytes. The surrounding tissue environment vitiliginous skin, especially keratinocytes, could play role recovering down-regulated non-lesional that lead return proliferation, differentiation melanogenesis via up-regulation SCF. melanocyte-related adhesion molecules (E-cadherin, DDR1, GPNMB HSD17β1) those Those vivo friction perilesional induces detachment living basal layer followed by transepidermal migration eventual death detached bFGF2 both based on confocal laser immunofluorescence microscopy. These results suggest plays an active development participates its pathogenesis might be involved pathogenetic chain events leading vitiligo.